Figure 4
Separation of Pr74Gag cleavage products by RP-HPLC. (A) Gag subdomains of purified HERVK113_ GagProPol VLPs were chromatographically separated by RPHPLC on an RP-C8 column. Proteins were eluted by an increasing acetonitrile gradient. Fractions were taken every minute and the eluted material was detected by UV absorption at 280 nm (AU, adsorption units). (B) The proteins in the fractions with the major peaks (fraction 34, 43, 45, 56 and 59) were analyzed by Western blot using the antisera against the presumed MA (left panel), p15 (central panel) and CA (right panel) domains.