Figure 3

HIV-1 transfer correlates directly with ps20 expression levels in primary CD4⁺ T cell clones. (A) Mean percentage of Gag⁺ dye-labelled ps20^low, ps20^inter and ps20^high primary target CD4⁺ clones 4 hours post co-culture with 40% Jwt ps20^inter 2044-infected donor cells at a T:D ratio of 1:0.2. Mean relative copy number of ps20 mRNA of each clone is given along the x-axis. (B) Correlation coefficient comparing the relative expression of ps20 in Clones 1,3,4,6,7,8 with their corresponding level of HIV-1 transfer 4 hours post co-culture with 40% Jwt ps20^inter 2044-infected donor cells at a T:D ratio of 1:0.2. (C) Clone 7 (ps20^high) was used as the target population and seeded at 1 × 10⁵ cells in the presence or the absence of 5 μg/ml of T-20 or 5 μM of RT-inhibitors (AZT+Lamimidine) for 1 hour prior to co-culture with 40% 2044-infected donor cells at T:D ratio of 1:0.2. The percentage of Gag⁺ target cells 48 hours post co-culture is shown. All data represent the mean of three replicate assays. Asterisks denotes statistically significant data as calculated using an unpaired t-test (Figure A), a two-tailed non-parametric Spearman's r correlation (Figure B) or paired t-test (Figure C). *P ≤0.05; **P ≤0.01; ***P ≤0.001; ****P ≤0.001.