Figure 7
From: Antiviral activity of α-helical stapled peptides designed from the HIV-1 capsid dimerization domain
NYAD-201 inhibits HIV-1 but not EIAV particle production. (A) 293T cells were transfected with pNL4-3 and 5 h after transfection treated with indicated concentrations of NYAD-201 or NYAD-233 for 18 h. Cells were then metabolically labeled with [35S]Met/Cys for 2 h. Cells were lysed and virions were collected by ultracentrifugation. Cell and virus lysates were immunoprecipitated with HIV-Ig and subjected to SDS-PAGE; protein bands were quantified by phosphorimager analysis. HIV-1 release efficiency was calculated as the amount of virion-associated p24 relative to total (cell + virion) Gag. (B) 293T cells were transfected with CMV-driven vectors expressing EIAV (pPRE-Gag) or HIV-1 (pCMVdeltaR8.2/PR-) Gag and treated with NYAD-201 at indicated concentrations. After 18 h, peptide-treated cells were metabolically labeled with [35S]Met/Cys for 2 h (HIV-1) or 5 h (EIAV) and immunoprecipitated with HIV-Ig or anti-EIAV antiserum. The release efficiency was calculated as the amount of virion-associated Pr55Gag to total Pr55Gag in cells and virions. P values were calculated by Student's t-test, with P < 0.01 considered significant. N = 3, ± SD.