Table 3 qPCR validation of differentially expressed genes
Gene Symbol | Accesion No. | Description | Fwd Primer | Fwd Primer Seq | Rev Primer | Rev Primer Seq | Paired Comparison | Cell Type | FC qPCR | FC MA |
|---|---|---|---|---|---|---|---|---|---|---|
KLRD1 | NM_002262.2 | killer cell lectin-like receptor subfamily D, member 1 | KLRD1L | gtgggagaatggctctgc | KLRD1R | tttgtattaaaagtttcaaatgatgga | BDLvsLTNP | CD8 | 2.5 | 2.1 |
IRS2 | NM_003749.2 | insulin receptor substrate 2 | IRS2L | tgacttcttgtcccaccactt | IRS2R | catcctggtgataaagccaga | BDLvsVIR | CD8 | 3.8 | 2.7 |
GBP1 | NM_002053.2 | guanylate binding protein 1, interferon-inducible | GBP1L | aggccacatcctagttctgc | GBP1R | tccaggagtcattctggttgt | BDLvsVIR | CD8 | -2.5 | -2.4 |
ACTA2 | NM_001613.1 | actin, alpha 2, smooth muscle, aorta | ACTA2L | ctgttccagccatccttcat | ACTA2R | tcatgatgctgttgtaggtggt | BDLvsVIR | CD8 | -1.3 | -2.2 |
ATP6V1D | NM_015994.2 | ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D | ATP6V1DL | ttttcactagctgaagccaagtt | ATP6V1DR | gcgctttattgacattttggat | VIRvsLTNP | CD8 | 2.0 | 2.8 |
BAG3 | NM_004281.3 | BCL2-associated athanogene 3 | BAG3L | cagccagataaacagtgtggac | BAG3R | agaggcagctggagactgg | VIRvsLTNP | CD8 | -1.5 | -2.4 |
ACTA2 | NM_001613.1 | actin, alpha 2, smooth muscle, aorta | ACTA2L | ctgttccagccatccttcat | ACTA2R | tcatgatgctgttgtaggtggt | VIRvsLTNP | CD8 | 4.3 | 2.8 |
PSMB2 | NM_002794.3 | proteasome subunit, beta type, 2 | PSMB2L | agagggcagtggaactcctt | PSMB2R | gaaggttggcagattcagga | VIRvsLTNP | CD8 | 1.3 | 2.3 |
PSMA5 | NM_002790.2 | proteasome subunit, alpha type, 5 | PSMA5L | tgaatgcaacaaacattgagc | PSMA5R | ttcttcctttgtgaacatgtgg | VIRvsLTNP | CD8 | 2.7 | 2.7 |
C1QB | NM_000491.3 | complement component 1, q subcomponent, B chain | C1QBL | ggcctcacaggacaccag | C1QBR | ccatgggatcttcatcatcata | BDLvsVIR | CD4 | -4.8 | -4.8 |
C1QC | NM_172369.3 | complement component 1, q subcomponent, C chain | C1QCL | aaggatgggtacgacggact | C1QCR | ttctgccctttgggtcct | BDLvsVIR | CD4 | -5.6 | -4.1 |
SERPING1 | NM_000062.2 | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1, | SERPING1L | ctccttacccaggtcctgct | SERPING1R | ggatgctctccaggtttgtt | BDLvsVIR | CD4 | -5.0 | -2.6 |
C1QB | NM_000491.3 | complement component 1, q subcomponent, B chain | C1QBL | ggcctcacaggacaccag | C1QBR | ccatgggatcttcatcatcata | VIRvsLTNP | CD4 | 6.1 | 6.0 |
C1QC | NM_172369.3 | complement component 1, q subcomponent, C chain | C1QCL | aaggatgggtacgacggact | C1QCR | ttctgccctttgggtcct | VIRvsLTNP | CD4 | 7.3 | 4.4 |
SERPING1 | NM_000062.2 | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1, | SERPING1L | ctccttacccaggtcctgct | SERPING1R | ggatgctctccaggtttgtt | VIRvsLTNP | CD4 | 5.3 | 2.8 |