Figure 5
Panel A: Competition experiment between GNA maize and GNA for binding to HIV-1 III B gp120 (chip density 400 RU ~ 3.3 fmol). 20 ÎĽM GNAmaize were injected (time point 1, green and magenta), followed after 2 minutes by injection of 20 ÎĽM GNAmaize (time point 2) in the absence (green) and presence of 5 ÎĽM GNA (magenta). Also 5 ÎĽM GNA (time point 1, red and blue) were injected followed by injection of 5 ÎĽM GNA (time point 2) in the absence (red) and presence of 20 ÎĽM GNAmaize (blue). In Panel B, the competition experiment was performed between the plant lectins GNA and GNAmaize, and the mAb 2G12 for binding to immobilized IIIB gp120. 20 ÎĽM GNAmaize (green and magenta) and 5 ÎĽM GNA (red and blue) were injected as such (first 120 sec), followed by an additional injection of 3 ÎĽM 2G12 (next 120 sec) (in the continued presence of GNAmaize [magenta] or GNA[blue]). In Panel C, 3 ÎĽM of 2G12 were injected (time point 1, red, blue and green) followed after 120 sec by an additional injection of 20 ÎĽM GNAmaize (blue), 5 ÎĽM GNA (green) or by no injection (red) (time point 2) for another 120 seconds, in the continued presence of 3 ÎĽM 2G12.