Background
Pakistan is in the concentrated phase of HIV epidemic for the last five years. The HIV prevalence among injecting drug users and hijra sex workers has been reported by second generation surveillance to be more than 5%. Considering the risk of HIV transmission to the spouses and children of the high risk groups including migrant workers, the National AIDS Control Programme established six comprehensive prevention of parent to child transmission (PPTCT) sites linked with HIV treatment centres in tertiary hospitals. The objective of this study was to determine the HIV prevalence among antenatal clinic (ANC) attendees at two tertiary care hospitals and correlate risk assessment questionnaire with HIV diagnosis.