Among 394 subjects, the prevalence of HIV infection was 5.1%, with 6.9% among MSWs in parks (PMSW), 11.3% among MSWs in family clubs(FMSW, a kind of home-based brothels holding small group of MSWs <= 15) and 1.7% among MSWs in entertainment venues (EMSW, holding big group of MSWs >15). Syphilis prevalence was 14.2%, with 25.0% in PMSWs, 17.6% in FMSW and 10.6% in EMSWs. Both HIV and syphilis prevalence were significantly different among different type of MSWs. Independent PMSWs and small grouping FMSWs had a higher proportion of self-identifying as homosexual/gay and fewer female sex partners, elder or higher polarization in age. In addition, a higher proportion of MSWs was found coming from provinces with higher HIV prevalence, and lower coverage by HIV-related education program and relevant services.