Figure 3

CD8⁺ lymphocyte Cyclin E production in FIV⁺ and FIV^- cats following CD4⁺CD25⁺ co-culture. Cyclin E production begins during late G₁ phase and peaks during early to mid S phase. CD8⁺ LN cells from either FIV⁺ (left) or FIV^- (right) cats were either untreated (first column) or co-cultured with autologous CD4⁺CD25⁺ Treg cells (second column). Shown above is a representative blot for experiments from FIV⁺ (n = 4) and FIV^- (n = 2) cats. The mean cyclin E production was increased greater than two-fold in FIV⁺ cats following a twelve hour CD4⁺CD25⁺ co-culture and decreased approximately one-fold in FIV^- cats.