Figure 2

CD8⁺ lymphocyte Cyclin D ₃ production in FIV⁺ and FIV^- cats following CD4⁺CD25⁺ co-culture. Cyclin D₃ typically assembles with CDK4 or CDK6 during mid G₁ phase and reaches maximal production during late G₁/early S phase. CD8⁺ LN cells from either FIV⁺ (left) or FIV^- (right) cats were either untreated (first column), or co-cultured with autologous CD4⁺CD25⁺ Treg cells (second column). Shown above is a representative blot for experiments from FIV⁺ (n = 4) and FIV^- (n = 2) cats. In both FIV⁺ and FIV^- control cats, the mean cyclin D3 production was reduced following a twelve hour incubation with CD4⁺CD25⁺ Treg cells.