CD8+ lymphocyte Cyclin D
production in FIV+ and FIV- cats following CD4+CD25+ co-culture. Cyclin D3 typically assembles with CDK4 or CDK6 during mid G1 phase and reaches maximal production during late G1/early S phase. CD8+ LN cells from either FIV+ (left) or FIV- (right) cats were either untreated (first column), or co-cultured with autologous CD4+CD25+ Treg cells (second column). Shown above is a representative blot for experiments from FIV+ (n = 4) and FIV- (n = 2) cats. In both FIV+ and FIV- control cats, the mean cyclin D3 production was reduced following a twelve hour incubation with CD4+CD25+ Treg cells.