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Figure 8 | Retrovirology

Figure 8

From: The membrane-spanning domain of gp41 plays a critical role in intracellular trafficking of the HIV envelope protein

Figure 8

Intracelluar distribution of Tac-gp41MSD chimera. The influence of MSD in transport of Tac proteines. Endoplasmic reticulum (ER) (A to C) and Golgi (D to I) regions were visualized by fluorescence protein-conjugated ER or Golgi marker proteins (shown in green). Halo tagged Tac-WT (A, D and G), Tac-gp41WT (B, E and H) and Tac-gp41 695+2A Env (C, F and I) were stained by anti-Halo antibody, anti-rabbit Ig and Alexa Fluor (shown in red). The close-up of the Golgi area was shown in G to I. Nuclei of cells were stained with Hoechst 33258 (shown in blue).

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