Figure 8

Foci morphology of infectious virus derived from pseudotyping NSCs. A. Representative foci (boxed areas) from cells infected with supernatants from CasE+4070A-NSCs stained for CasBrE Env showing non-contiguous foci morphologies indicative of defective replication incompetent virus. No contiguous 697 foci were noted under conditions where single-hit infection predominated, indicating that replication competent recombinant CasBrE viruses did not readily arise from CasE+4070A-NSCs or CasES+4070A-NSCs. B. Representative foci from fibroblasts infected with CasE+4070A supernatants stained with monoclonal antibody 83A25 which recognizes both 4070A and CasBrE Envs. This image illustrates both non-contiguous and contiguous foci morphologies, indicative of defective and replication competent viruses, respectively.