Figure 3

Myristoylation defective Nef does not exhibit enhanced oligomerization. (A, B) The abilities of untagged SF2Nef to associate with tagged SF2-HFNef or SF2-HFNefG2A in membrane and soluble fractions were compared using the immunoprecipitation assay. The co-immunoprecipitated SF2Nef is indicated by an arrow marked "U" to the right of lane 12 (lanes 11 and 12). The total tagged Nef is indicated by an arrow marked ''T''. (A), Membrane-bound Nef. (B), Cytosolic Nef. (C) Data from additional experiments were combined to assess the FDs for the binding of SF2Nef to SF2-HFNef and SF2-HFNefG2A. Analysis was performed as in Figure 2.