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Figure 4 | Retrovirology

Figure 4

From: Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5α-mediated restriction

Figure 4

Western blot analysis of CA and CypA in particles of GH123, SIVmac239 and their derivatives. Viral particles from HIV-1 NL43, HIV-2 GH123, SIVmac239, and their derivatives were purified by ultracentrifugation through a 20% sucrose cushion. A total of 120 ng of p24 of HIV-1, p25 of HIV-2 GH123 derivatives or p27 of SIVmac239 derivatives was applied for gel electropholesis. Cyp A (upper panel) and CA (lower panel) were visualized by Western blotting (WB) using an anti-CypA antibody and serum from a SIV-infected monkey, respectively.

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