Env incorporation into Wt-HIV and HIV-Env-Tr712 virion. Producer H9 (np) cells had been weakly infected (less than 20% infection level). A. Western blot: the top part of the filter has been probed with gp120 antibodies, the middle part with gp41 mAb, Chessie 8 against the Env C-terminal tail (truncated in HIV-Env-Tr712) and the bottom part with p24 mAb. B. Average gp120 incorporation into HIV-Env-Tr712 virions in comparison to HIV-Wt (from 3 independent experiments: individual values 37%, 118%, 83%).