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Figure 4 | Retrovirology

Figure 4

From: The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro

Figure 4

Steady-state DNA synthesis by HIV-1 RT in the presence of NusA-HuR. (A) HIV-1 RT DNA synthesis on a heteropolymeric RNA template corresponding to the HIV-1 sequence of (-) strong stop DNA. The 18 nucleotide DNA oligonucleotide primer is complementary to the HIV-1 tRNALys3 primer binding site in the RNA template. The reaction was carried out for 2, 5, 10, 30 60 min, respectively. (B) HIV-1 RT DNA synthesis carried out on an ARE containg T/P substrate. The sequences of the T/P is indicated with the ARE in bold and underlined. Binding of NusA-HuR to this T/P is observed (left-hand autoradiograph). However, the efficiency of HIV-1 RT DNA synthesis is not significantly affected (right-hand autoradiograph). The reaction times for DNA synthesis were 0, 1, 2, 3, 4, 5, 7.5, 10, 15, 20 and 30 min, respectively.

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Retrovirology

ISSN: 1742-4690

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