Figure 4
From: Vpu-dependent block to incorporation of GaLV Env into lentiviral vectors
Representative Western blot and quantitative analysis of signal density on blots from three independent experiments analyzing GaLV TM levels in lysates of 293T cells co-transfected with GaLV Env, pHR' and indicated derivatives of R8.2 or R8.91, with (+) expression of Vpu in trans , from plasmid CΔEvpu. Immunological detection of HIV-1 p24 and actin levels were included as controls.