Figure 6

Conferral of resistance to neutralisation by homologous plasma following V5 loop exchange. GL8 Env incorporating non-coding mutations that introduced restriction enzyme sites facilitating V5 loop exchange (GL8 "ABSN") was compared with three chimaeric Envs in which the V5s of B14, 19 and 28 had been introduced, or the parent B14, B19 and B28 Envs. HIV(FIV) luciferase pseudotypes bearing each Env were incubated with 1:10, 1:100, 1:1000, 1:10,000 or 1:100,000 dilutions of A613 plasma and plated onto CLL-CD134 cells. The chimaeric Envs bearing the V5s of B14, B19 and B28 recapitulated the neutralisation-resistant phenotype of the parent Envs. Each point represents the mean (+/- SE) of three replicates and is typical of at least two independent experiments.