Figure 3

Replication kinetics of viruses containing mutations in SP1 residues 6-8. Jurkat T cells were transfected with WT pNL4-3 and the panel of SP1 mutant derivatives and cultured either without BVM or in 50 ng/ml or 1 μg/ml BVM. Cells were split every 2 days, and supernatants were reserved at each time point for RT analysis. All originally introduced mutations were maintained. The grey boxes indicate those cultures in which an additional mutation is acquired; both the introduced and the acquired mutations are indicated. The results shown are representative of at least 2 independent experiments. Results from the repeat experiment are described in the text.