Figure 3

(A) IN-BAD and LEDGF/p75 co-immunoprecipitation from extracts of 293 cells mock-transduced (Mock) or transduced with the IN-BADv (293 IN-BADv), analysed by Western blotting with anti-LEDGF/p75 and anti-IN antibodies (1 minute exposure). (B) PCR detection of viral DNA in streptavidin capture (top) and LEDGF/p75 immunoprecipitates (bottom). 293 cells were transduced with the IN-BAD vector (293 IN-BADv) or mock transduced (Mock) in the absence or presence of AZT. Streptavidine capture or LEDGF/p75 co-immunoprecipitation were performed on cell lysates, and vector DNA was detected using PCR with the MH531 and MH532 primers [17]. The absence of amplification in the presence of AZT indicates that only neo-synthesized DNA was detected.