Figure 1
From: Changes in the accessibility of the HIV-1 Integrase C-terminus in the presence of cellular proteins
Fusion of the Biotin Acceptor Domain (BAD) to the IN C-terminus does not affect particle production, cDNA synthesis, and integration. (A) Amino acid sequence at the C-terminus of IN-BAD, in the context of a p8.74 derived gagpol expression construct. (B) Comparison of vector titres obtained with IN-BAD and IN-WT. Data represent the mean ± SD of GFP titres measured on HCT116 cells from three independent productions. (C) Kinetics of HIV-1 vector DNA synthesis during vector transduction of HEK 293 cells (30 ng of p24gag/106 cells) with or without AZT, analysed by quantitative PCR. (D) Amounts of integrated provirus. Data in C and D represent the mean ± SD of three independent transductions.