Assay/Application | Oligonucleotide | Sequence | Amplicon size (bp) | Nucleotide position (bp) |
---|---|---|---|---|
TaqMan® PCR assay |  |  |  |  |
   env variant1 | Forward | GAT CCG GAC CGA CCA TAA TTA A | 105 | 1,912 - 1,933 |
 | Probe | TGT ATG ATT CCA TTT AGT CCC6 |  | 1,935 - 1,955 |
 | Reverse | ACA CCA CTG CAG TAG CTG GCT AA |  | 2,017 - 1,995 |
Production of standard | Â | Â | Â | Â |
   FeLV-A/Glasgow-12 | Forward | TGG GGC CAA AGG GAA CAC AT | 598 | 456 - 475 |
 | Reverse | GTT ACC TAA GAT TGC AAT CCC TTC G |  | 1,054 - 1,030 |
   env variant3 | Forward5 | CCT ATG GCT CAC TTC TTT GAT ACT GAT ATC TCT A | 2,6647 | 5,617 - 5,650 |
 | Reverse | TTA TAG CAG AAA GCG CGC G |  | 8,281 - 8,263 |
In situ hybridization4 | Â | Â | Â | Â |
   p27 | Forward | TAC GCC TTT ATC GCC AGT TG | 342 | 1,840 - 1,859 |
 | Reverse | ATC TTT CTT CCC TTT CCT CTG G |  | 2,181 - 2,160 |
   gp70 | Forward | AGG GAT TGC AAT CTT AGG TA | 219 | 6,952 - 6,971 |
 | Reverse | TTA CAG GCC CAA TAG GTG |  | 7,170 - 7,153 |