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Table 3 Percentage of Gag CM9 positive cells quantified by imaging analysis of in situ stained lymphoid tissue sections.

From: In situ detection of Gag-specific CD8+cells in the GI tract of SIV infected Rhesus macaques

ID No

Specimen

Total cells/mm2

CD8+cells/mm2

Gag CM9+cells/mm2

% Gag CM9 of CD8+cells

RM 1

Spleen

16 678 (± 2 032)

2 399 (± 386)

164 (± 39)

6.80 (± 0.6)

RM 2

Spleen

20 789 (± 3 552)

2 048 (± 627)

198 (± 70)

9.59 (± 2.0)

RM 3

Spleen

20 276(± 3 877)

2 694 (± 494)

206 (± 49)

7.78 (± 2.0)

RM 1

Submandibular LN

31 340 (± 4 076)

3 333 (± 511)

170 (± 11)

5.21 (± 1.1)

RM 2

Submandibular LN

20 789 (± 3 552)

4 885 (± 1 134)

255 (± 78)

5.16 (± 0.6)

RM 3

Submandibular LN

18 754 (± 3 317)

3 684 (± 232)

122 (± 22)

3.31 (± 0.5)

RM 1

Mesenteric LN

23 330 (± 3 458)

6 876 (± 1 225)

262 (± 26)

3.91 (± 1.0)

RM 2

Mesenteric LN

17 975 (± 2 866)

2 969 (± 685)

183 (± 48)

6.51 (± 2.8)

RM 3

Mesenteric LN

21 588(± 3 470)

6 476 (± 1 163)

200 (± 42)

3.26 (± 1.4)

RM 1

Ileum

ND

ND

ND

ND

RM 2

Ileum

6 812 (± 2 603)

701 (± 7)

21 (± 3)

2.97 (± 0.4)

RM 3

Ileum

6 793 (± 1 246)

629 (± 206)

15 (± 5)

2.43 (± 0.3)

RM 1

Colon

10 049 (± 4 193)

1 145 (± 135)

77 (± 16)

6.74 (± 0.9)

RM 2

Colon

11 814 (± 5 130)

2 287 (± 1 424)

76 (± 52)

3.10 (± 0.5)

RM 3

Colon

11 126 (± 4 207)

888 (± 160)

42 (± 9)

4.80 (± 0.9

  1. The average total cell number/mm2, CD8+ cells/mm2, Gag CM9+ cells/mm2 and percentage of Gag CM9 Qdot 655 multimer positive cells in the CD8+ cell population was calculated by using the image J particle counting program. The standard deviation is shown in brackets. Staining was performed at least three times for each specimen and a total tissue area of 4.6 × 106 μm2 was counted for each specimen. LN; lymph node and ND; not done due to lack of material.