Figure 2

Staining Patterns of Gag CM9. A) Fluorescence image of a lymphocyte from an SIV infected Mamu-A*01 positive RM stained with Gag CM9 Qdot 655 multimer (red) and CD8 (green). B) Fluorescence image of a lymphocyte from an SIV infected Mamu-A*01 positive RM stained with Gag CM9 APC Tetramer (red) and CD8 (green). C) Confocal fluorescence image of a colon tissue section from an SIV infected Mamu-A*01 positive RM stained with Gag CM9 Qdot 655 multimer (Red), CD8 (green) and Dapi (blue). Scale bar = 10 μm. D) A magnified view of the region indicated in panel C. Cells stained with Qdot 655 multimer or APC tetramer show a punctate Gag CM9 staining pattern. All images were acquired with a 100×/1.4 oil immersion objective and further deconvolved. E-H) Volocity (Improvision) software was used to generate a surface model of the CD8⁺Gag CM9⁺ cells shown in panel A (E, F) and panel D (G,H). E, G: CD8 (green), Gag CM9 Qdot 655 multimer (red); F, H: overlap (yellow) of CD8 and Gag CM9 Qdot 655 multimer staining.