Figure 6

Hepcidin increases intracellular iron in THP-1 but not in CEM T-cells. THP-1 cells (A) or CEM T-cells (B) were treated as indicated with 0.5 μM hepcidin for 3 hours and then 25 μM Ferric Sulfate for 1 hr. Then the cells were loaded with 0.1 μM calcein-AM for 10 min at 37°C, and treated with 30 μM SIH. Fluorescence of calcein was measured before and during SIH treatment. Results are presented as fractional fluorescence (F-Fi)/Fi, where Fi = fluorescence in the presence of quencher at time 0, and F = fluorescence at given time.