Volume 6 Supplement 1

Fifth Dominique Dormont International Conference. Host-Pathogen Interactions in Chronic Infections

Open Access

Perinatal transmission of Cytomegalovirus (CMV) in children born to HIV positive and negative women in Cameroon

  • Anfumbom Kfutwah1,
  • Mathurin Tejiokem1,
  • Albert Faye2,
  • Martial Yonga1,
  • Josiane Warszawski3,
  • Stephane Blanche4,
  • Claire Rekacewicz5,
  • Francis Ateba6,
  • Jean Ndongo6,
  • Georgette Guemkam6,
  • Chantal Same-Ekobo7,
  • Christine Rouzioux4,
  • Marianne Leruez-Ville4,
  • Pascal Boisier1 and
  • Dominique Rousset1
Retrovirology20096(Suppl 1):O23

https://doi.org/10.1186/1742-4690-6-S1-O23

Published: 22 July 2009

Background

Cytomegalovirus (CMV) infection is considered as a risk factor in disease progression in HIV co-infected infants. The objective of this study was to evaluate the perinatal transmission of CMV in children born to HIV positive women compare to HIV negative women and to evaluate the prevalence of CMV in HIV infected infants.

Methods

Children born to HIV positive and negative women who participate in ANRS 12140 survey (Pediacam) in Yaoundé (Cameroon) were included in this study. Whole blood samples were collected from the children at the moment of inclusion into Pediacam. DNA was extracted using the Qiagen blood extraction kit and CMV DNA was quantified using a real time in house CMV PCR (ABI Prism 7700, Applied Bio systems). Two groups of HIV infected children diagnosed before 6 months were included: children followed since birth with early diagnosis (group I; n = 17) and those not followed since birth and diagnosed generally later (group III; n = 29). There were two groups of HIV uninfected children followed since birth: those born to HIV-infected mothers (group II; n = 11) and those born to seronegative mothers (group IV; n = 12).

Results

Among the 69 children who participated in this study (median age; 15 weeks [range 3–34 weeks]) 31 (45%) had a positive CMV detection. CMV prevalence was 31 and 65% in groups I and III respectively and 0 and 64% in groups II and IV. All infants of group IV were breastfed while the other groups consisted of children who were either breast-fed or bottle-fed. As expected, CMV prevalence was higher in the breastfed children 21/33 (66%) than in the bottle-fed children 7/34 (21%) (P < 10-4) (Information not available for 2 children.)

There was a 30% increase in CMV prevalence among HIV infected children compared to non infected children in both the breastfed group (81.2 % versus 50%) and the bottle fed group (29% versus 0%). The median CMV viral load was 3.6 log copies/ml [range 1.2 to 6.5]. Viral load was higher in group III (4.6 log copies/ml) compared to groups I, II, and IV (3.1, 0, and 2.8 log copies/ml respectively) (P = 0.01). Among the infants of group III who presented with non specific symptoms, CMV was detected in 12/16 (75%). Group III presented subtle differences in CD4 cell percentages between CMV infected and non infected children 23% vs. 31%.

Conclusion and perspective

This preliminary study showed a high incidence of CMV infection in HIV infected infants in Cameroon. As previously described, breastfeeding and HIV transmission irrespective of mode of feeding were associated with CMV infection. Most of the HIV symptomatic children were infected with CMV. This co-infection may be a major factor of morbidity/mortality in children with severe HIV disease in the African setting.

Authors’ Affiliations

(1)
Centre Pasteur
(2)
Hôpital Robert Debré, Service de Pédiatrie Générale
(3)
Unité Inserm U822, CHU de Bicêtre
(4)
Hôpital Necker Enfants Malades
(5)
ANRS
(6)
Centre Mère et Enfant de la Fondation Chantal Biya – Maternité Principale Hôpital Central de Yaoundé
(7)
Centre Hospitalier d'Essos à Yaoundé

Copyright

© Kfutwah et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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