Genetic characterization of the complete genome of a highly divergent simian T-lymphotropic virus (STLV) type 3 from a wild Cercopithecus mona monkey

Table 1 PCR primer pairs^1,2 used to amplify overlapping regions of the STLV-3d(Cmo8699AB) genome

Fragment	Region	Primer set	Primer	Sequence (5'-->3')	Primer	Sequence (5'-->3')	bp
B	LTR-gag	Outer	P5LF5	TCA ACC TTT TCT CCC CAA CGC CCT	P3GR6	AYT GGR GGC TRC CWG GGG CGG AAG	954
		Inner	P5LF6	GCA CCT TCG CTT CTC CTG TCC TGG	P3GR7	GRT AGG GYG GAG GCT TTT GRG GGT	692
C	gag-pol	Outer	P5GF1	GTG CCG CCA ACC CCA TCC CCA AGG	PGPOLR1	GGY RTG IAR CCA RRC IAG KGG CCA	2687
		Inner	P5GF2	AAA GGG CTA GCA ATT CAC CAC TGG	P3GR1	GAT AGG GTT ATT GCC TGG TCC TTG ATA	1770
D	pol	Outer	8699GF20	ACC CCC CCA GTA AGC ATC CAG GCG	PGPOLR1	GGY RTG IAR CCA RRC IAG KGG CCA	1360
		Inner	8699GF21	AGA TGT CCT CCA GCA ATG CCA AAG	PGPOLR2	GRY RGG IGT ICC TTT IGA GAC CCA	992
E	pol-env	Outer	7867GPF2	TCC ACA GAA AAA ACC CAA TCC ACT	8699ETF2R	GGG CAG TAG CAA TGG GAC CAA GGA	2864
		Inner	7867GPF3	CAC TCC TGG TCC CAT ACA CTT TCT CGG	8699ETF1R	GGT GGG GCC TGT GTA GTT TGG GAG	2556
F	env-tax	Outer	7867EF1	AAA GTC TAA ACC CTC CAT GCC CAG	8699TR5	TTT GGT AGG GAT TTT TGT TAG GAA GG	2560
		Inner	7867EF2	TCC TTG TAT CTT TTT CCC CAT TGG	8699TR1	AAG GTA TTG TAG AGG CGA GCT GAC	2147

¹ The primers used to amplify tax and LTR overlapping regions (fragments A, G, H, I depicted in figure 1) are described elsewhere [24].
2. I = inosine; other letters are as defined by the IUPAC code.

ISSN: 1742-4690