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Table 1 PCR primer pairs1,2 used to amplify overlapping regions of the STLV-3d(Cmo8699AB) genome

From: Genetic characterization of the complete genome of a highly divergent simian T-lymphotropic virus (STLV) type 3 from a wild Cercopithecus mona monkey

Fragment Region Primer set Primer Sequence (5'-->3') Primer Sequence (5'-->3') bp
B LTR-gag Outer P5LF5 TCA ACC TTT TCT CCC CAA CGC CCT P3GR6 AYT GGR GGC TRC CWG GGG CGG AAG 954
   Inner P5LF6 GCA CCT TCG CTT CTC CTG TCC TGG P3GR7 GRT AGG GYG GAG GCT TTT GRG GGT 692
C gag-pol Outer P5GF1 GTG CCG CCA ACC CCA TCC CCA AGG PGPOLR1 GGY RTG IAR CCA RRC IAG KGG CCA 2687
   Inner P5GF2 AAA GGG CTA GCA ATT CAC CAC TGG P3GR1 GAT AGG GTT ATT GCC TGG TCC TTG ATA 1770
D pol Outer 8699GF20 ACC CCC CCA GTA AGC ATC CAG GCG PGPOLR1 GGY RTG IAR CCA RRC IAG KGG CCA 1360
   Inner 8699GF21 AGA TGT CCT CCA GCA ATG CCA AAG PGPOLR2 GRY RGG IGT ICC TTT IGA GAC CCA 992
E pol-env Outer 7867GPF2 TCC ACA GAA AAA ACC CAA TCC ACT 8699ETF2R GGG CAG TAG CAA TGG GAC CAA GGA 2864
   Inner 7867GPF3 CAC TCC TGG TCC CAT ACA CTT TCT CGG 8699ETF1R GGT GGG GCC TGT GTA GTT TGG GAG 2556
F env-tax Outer 7867EF1 AAA GTC TAA ACC CTC CAT GCC CAG 8699TR5 TTT GGT AGG GAT TTT TGT TAG GAA GG 2560
   Inner 7867EF2 TCC TTG TAT CTT TTT CCC CAT TGG 8699TR1 AAG GTA TTG TAG AGG CGA GCT GAC 2147
  1. 1 The primers used to amplify tax and LTR overlapping regions (fragments A, G, H, I depicted in figure 1) are described elsewhere [24].
  2. 2. I = inosine; other letters are as defined by the IUPAC code.