Figure 3
From: Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase
WT and mutant IN 3' processing activities. (A) Polyacrylamide gel images reveal the migration positions of labeled 30-mer DNA substrate (S), cleaved pGTOH dinucleotide, as well as pTOH mononucleotide. The reactions loaded in lanes 1 and 17 contained exonuclease III in place of IN, whereas lanes 2 and 18 omitted IN. The reactions in the remaining lanes contained the indicated IN proteins. (B) Mutant 3' processing activities plotted as percentage of WT IN function. Results are mean ± SEM for two (HIV-1/2 chimera) to four (all other mutants) independent experiments.