Figure 3

Analyses of E36 cells. Panel A. Susceptibility of E36 hamster cells expressing different Xpr1 receptors to LacZ pseudotypes of X/PMVs. Receptor genes cloned from NIH 3T3 cells (Xpr1ⁿ) and M. pahari cells (Xpr1^p) were tested along with the indicated chimeras and mutants. Titers represent the averages of 3 or more experiments and are given as the number of LacZ positive cells/100 μl with SD. E36 cells show trace infectivity with CAST-X (<1). Panel B. Western blot analysis of the expression of E36 cells transfected with the indicated Xpr1 mutants. Expression was detected using an anti-V5 antibody (top). The lanes on the right were cut from the same photograph of a single Western blot.