Figure 6

The transforming activities of the Tax1 chimeric proteins with Tax2B. (A, C) CTLL-2 cells were infected with lentiviruses encoding the indicated Tax genes in the presence of IL-2. At 48 hours after infection, the cells (1 × 10³, 3 × 10³, and 1 × 10⁴ cells/well) were cultured in 96-well plates without IL-2. After 4 weeks of culture, the wells containing the outgrowing cells were counted using light microscopy. The number of positive wells is shown; the maximum number was 96. (A, C) The cell lysates prepared at 48 hours after infection (B, D) were characterized by a Western blot analysis probed with the anti-Tax1 antibody. The data are representative of three independent experiments. *The P values were < 0.001.