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Figure 2 | Retrovirology

Figure 2

From: Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2

Figure 2

Tax1 and its mutants induce nuclear localization of p52. Jurkat cells were infected with lentiviruses encoding the indicated proteins. The cytoplasmic and nuclear lysates were prepared 48 hours after infection, and the amounts of p100, p52, Tax, α-Tubulin, and Sp1 in the cytoplamic and nuclear lysates were measured by Western blotting analysis using anti-p100, anti-Tax1, anti-α-Tubulin, and anti-Sp1 antibodies. EGFP was translated from a bicistronic transcript encoding the tax gene, and the infection level (%) was normalized by EGFP expression of the cells infected with the indicated lentiviruses. The anti-Tax1 antibody could not recognize Tax2B protein.

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Retrovirology

ISSN: 1742-4690

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