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Figure 1 | Retrovirology

Figure 1

From: Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2

Figure 1

NF-κB2/p100 processing activities of Tax1 chimeric proteins with Tax2. (A) The structure of Tax1, Tax2B, and their mutants, and the boundary amino acids of the chimeras are indicated. (B) Jurkat cells were infected with lentiviruses encoding the indicated proteins. The cell lysates were prepared 48 hours after infection, and the amounts of p100, p52, Tax and α-Tubulin in the lysates were measured by Western blotting analysis using anti-p100, anit-Tax1, and anti-α-Tubulin antibodies. EGFP was translated from a bicistronic transcript encoding the tax gene, and the infection level (%) was normalized by EGFP expression of the cells infected with the indicated lentiviruses. The anti-Tax1 antibody could not recognize Tax2B protein.

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Retrovirology

ISSN: 1742-4690

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