Table 1 DNA oligonucleotides used in the present study
From: Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein
Oligo DNA | Sequence and position on Tat mRNA |
|---|---|
LeaderR sense EcoRI | 5' GAATTCGGTCTCTCTGGTTAGACCAGATC 3' (exon1 sense for Tat1 et Tat2: 1→) |
Leader rev1 | 5' TATTCTGCTATGTCGACACCCAATTCAGTCGCCGCCCCTCG 3' (exon1 reverse for Tat1:← 290) |
Leader rev2 | 5' GGATCTCTGCTGTCCCTGCAGTCGCCGCCCCTCG 3' (exon1 reverse for Tat2:← 290) |
Ex2Tat sense | 5' AATTGGGTGTCGACATAGCAGAATAGGCG 3' (exon2 sense for Tat1:290→ and for Tat2:343→) |
Ex2Tat rev | 5'GGGATTGGGAGGTGGGTTGCTTTGATAGAGAAGCTTGATGAGTCTGACTG3' (exon2: reverse for Tat1:← 558 and for Tat2:← 611) |
Ex'Tat sense | 5'CAGGGACAGCAGAGATCCAGTTTGGAAAGGACCAGCAAAGCTCCTCTGGAAAG 3' (exon':sense for Tat2:290→) |
Ex'Tat rev | 5' CTGCTATGTCGACACCCAATTCTTTCCAGAGGAGCTTTGCTG 3' (exon':reverse for Tat2:← 343) |
Ex3Tat sense | 5' ACCCACCTCCCAATCCCGAG 3' (exon3: sense for Tat1:558→ and for Tat2: 611→) |
Tatrev NotI | 5' TAATAATGCGGCCGCAGTACAGGCAAAAAGCAGCTGCTTATATGC 3' (exon3: reverse for Tat1:← 1718 and for Tat2:← 1770) |
PBSEcoRI sense | 5' TATATTAGAATTCGTGTGCCCGTCTGTTGTGTGACT 3' (exon1: sense for Tat1 and for Tat2:104 →) |
SDEcoRI sense | 5' ATATAAGAATTCCGAGGGGCGGCGACTG 3' (exon1: sense for Tat1 and for Tat2:274→) |
AUGEcoRI sense | 5' TATAATAGAATTCATGGAGCCAGTAGATCCTAGACTAGAG 3' (exon2: sense for Tat1:343 → and for Tat2:396 →) |
TatNcoI sense | 5' TAATATACCATGGGGTCTCTCTGGTTAGACCAGATC 3' (exon1: sense for Tat1 and for Tat2:1→) |
TatSmaI rev | 5' TATATACCCGGGAGTACAGGCAAAAAGCAGCTGCTTATATGC 3' (exon3: reverse for Tat1:← 1718 and for Tat2:← 1770) |
TatXbaI sense | 5' ATATATTCTAGAGGTCTCTCTGGTTAGACCAGATC 3' (exon 1:for Tat1 et Tat2:1→) |
TatXbaI rev | 5' TAATAATTCTAGAAGTACAGGCAAAAAGCAGCTGCTTATATGC 3' (exon3 reverse for Tat1:← 1718 and for Tat2:← 1770) |
(111)NcoI rev | 5' TATATACCATGGGGCACACACTACTTTGAGCACTCAAGG 3' (exon1:reverse:← 111) |
UTRTatNcoI rev | 5' TATATTACCATGGTTCTTGCTCTCCTCTGTCGAGTAACG 3' (exon2: reverse for Tat1:← 342 and for Tat2:← 395) |
(1-336)NcoI rev | 5' ATATATACCATGGCTCTCTCCTTCTAGCCTCCGC 3' (reverse: 5' to AUG of the RNAg) |
UTR2TatNcoI sense | 5' TAATATACCATGGGTGTGCCCGTCTGTTGTGTGACT 3' (exon1: sense for Tat1 and for Tat2:104 →) |
LTRPvuII sense | 5' TAATATACAGCTGTGGAAGGGCTAATTTGGTCCC 3' (sense for U3) |
LTRPvuII rev | 5' TATATTACAGCTGAGTACAGGCAAAAAGCAGCTGC 3' (reverse for U3) |