Figure 2

Differential expression of ALVE in brain and peripheral tissues of HWS and LWS chickens. Relative mRNA expression levels of env gene were measured using qRT-PCR with a primer pair b* shown in table 1 and figure 1B. A. Validation of differential expression of ALVE genes in cDNA microarray experiment. One-way ANOVA together with Newman-Keuls test as a post-hoc analysis was utilized. B. ALVE expression in peripheral tissues of HWS and LWS lines. Peripheral tissues were dissected from chickens on day 56 and the brain from chicks at hatch. N = 3 for each of HWS and LWS lines in all peripheral tissues and cDNA samples from five birds were pooled for the brain pools. H, HWS; L, LWS; M, males; F, females.