Table 1 Propagation of MMTV and MPMV transfer vectors RNA by homologous and heterologous proteins.

pDA024

Chimeric LTR, 5' region upto 400 bp of MMTV Gag, and SV-hyg^r

3,676 ± 196

< 1

-

-

pSS013

Chimeric LTR, 5' region upto 400 bp of MMTV Gag, and SV-EGFP

-

-

19

< 1

pND015

Same as DA024 but the putative MMTV ψ has been replaced with that of MPMV (5' UTR + 282 bp of Gag)

2

8

-

-

pND016

Same as DA024 but the putative MMTV ψ and PBS have been replaced with that of MPMV

< 1

7

-

-

pND017

Control MMTV vector in which putative MMTV ψ has been cloned back after creating NotI site at PBS/UTR junction

187 ± 33

ND

-

-

pND018

Control MMTV vector in which putative MMTV ψ and PBS have been cloned back after creating NotI site at U5/PBS junction

30 ± 4

ND

-

-

pKAL011

MPMV 5' region upto 282 bp Gag and SV-hyg^r

< 1

36,373 ± 3,972

-

-

pSS015

Same as KAL011 but has SV-EGFP instead of SV-hyg^r

-

-

< 1

41

pND011

Same as KAL011 but MPMV ψ has been replaced with that of MMTV (5' UTR + 400 bp of Gag)

< 1

3

-

-

pND012

Same as KAL011 but MPMV ψ and PBS have been replaced with that of MMTV

< 1

23

-

-

pND013

Control MPMV vector in which MPMV ψ has been cloned back after creating NotI site at PBS/UTR junction

ND

2813 ± 99

-

-

pND014

Control MPMV vector in which MPMV ψ and PBS have been cloned back after creating NotI site at U5/PBS junction

ND

1660 ± 111

-

-

pTR174

Control vector containing SV-hyg^rcassette and SIV 3'LTR as poly(A)

< 1

< 1

-

-

pAG001

Control vector containing SV-hyg^rcassette and FIV 3'LTR as poly(A)

< 1

< 1

-

-

Mock

No DNA (control)

<1

< 1

< 1

< 1