Figure 5
Restriction activities of M. leonina TRIMCyp on lentiviruses. (A) The indicated cells were infected with HIV-GFP-VSVG at the indicated infection unit (IU). IU = 0.1 or 0.5 denotes 10-fold or 2-fold dilution of HIV-GFP-VSVG viral stocks, respectively. Percentage of infected cells was counted 48 hours later by FACS analysis of GFP positive cells. The result is representative of three independent experiments. npmPBMC: northern pig-tailed macaque PBMC; huPBMC: human PBMC; rhPBMC: rhesus PBMC; MT-4: human T cell line MT-4; U937: human monocyte line U937; rh B: a Chinese rhesus macaque transformed B immortalized cell line. (B) The HA-tagged TRIMCyp cloned from northern pig-tailed macaque and owl monkey TRIMCyp were stably expressed in HeLa-T4 cells. The expression of the proteins was confirmed by Western blotting. (C) The cells were infected with HIV-2ROD or SIVmac239 virus at MOI (multiplicity of infection) = 0.02. Four days post-infection, the capsid p27 antigen levels in the culture supernatants were measured by quantitative ELISA assays. The result is representative of three independent experiments.