Figure 2

Suppression of HIV-1 release by exogenous hBST-2 in various cell lines. One microgram of pNL4-3 and pNL43-Udel was each co-transfected with (20 or 100 ng) or without (-) phBST-2 into several lines of cells as described in the Materials and Methods. The amount of wild-type (opened bars) or Vpu-deficient HIV-1 virion (bars filled in gray) released from HeLa, HEK293, Vero, Cos-7, D-17, PK-15, RSC, Mv.1.Lu, and QT6 was quantified by using TZM-bl cells, and the amount of HIV-1 released from CRFK and PtK2 cells was quantified by p24 ELISA. All experiments were performed in triplicate. Statistical significance (Student's t test) versus wild-type HIV-1 values is represented as follows: *, P < 0.05; **, P < 0.01. Error bars indicate standard deviations. n.d., not detectable.