Figure 1

Characterization of the studied cell population and overview of the experimental design. (A) PBMCs were subjected to magnetic-based CD4⁺ T cell negative selection. Percentages of CD4⁺ (left panel) and CD14⁺ cells (right panel) in the enriched population were estimated by flow cytometry immediately after selection. The right panel represents a worst-case scenario for contaminating CD14⁺ cells. (B) The studied cell population highly enriched in CD4⁺ T cells were infected with fully infectious eGFP-encoding viruses either lacking (NL4-3 wt) or bearing host-derived ICAM-1 (NL4-3 ICAM-1+) and the number of cells productively infected with HIV-1 (i.e. eGFP-positive) was estimated by flow cytometry at the listed days following infection. (C) A schematic representation of the experimental design used for the microarray studies is shown.