The reduction of proteasomal degradation increases VSV-G pseudotyped HIV-1 replication, but not replication of HIV-1 enveloped pseudotypes. VSV-G HIV-1 viral infection in lactacystin treated BeWo cells increases compared to untreated cells. The cells were exposed to the pseudotyped HIV-1 viruses (X4 and R5 pseudotypes, as well as the VSV-G pseudotype) and the level of infection in the cells was determined by the amount of luciferase activity detected in the cell lysates 96 hours post-infection. Results are expressed as the fold induction of luciferase activity as compared to uninfected cells. The results show mean of three independent experiments. Error bars indicate SEM. Statistical analyses were performed using Mann Whitney test.