Skip to main content
Figure 2 | Retrovirology

Figure 2

From: TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+T cells

Figure 2

De novo virus production in IM-MDDCs is affected by TLR2 and 4 engagement. (A) IM-MDDCs were either left untreated (mock) or stimulated for 2 hours with the listed TLR agonists. Thereafter, cells were washed twice and pulsed with NL4-3/Balenv. IM-MDDCs were either left untreated (left panel) or treated with EFV (right panel) before addition of TLR agonists. Supernatants were harvested at 3, 6 and 9 days post-infection (dpi) and the viral content was monitored by a p24 test. Data depicted represent the mean ± standard deviations of quadruplicate samples from a single donor and are representative of 3 different donors. (B) A similar experimental strategy was used except that cells were either left untreated or exposed to the listed TLR2 ligands. Data shown represent the mean ± standard deviations of quadruplicate samples from two different donors (3 dpi). (C) Cells were either left untreated (mock) or stimulated for 2 hours with the listed TLR2 agonists. Thereafter, cells were washed twice and pulsed with the clinical HIV-1 isolate 93TH054. Supernatants were harvested at 5 dpi and the viral content evaluated by a p24 test. The data shown represent the mean of quadruplicate samples from 2 different donors.

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us