HPLC analysis of G-NH
and the G-NH
-derived metabolite Met-X. HPLC chromatograms of dialysis solution (DS) after dialyzing 1 mM G-NH2 against porcine serum (PS) at 37°C (A) and at 4°C (B) or human serum at 37°C. The dialysis solutions were analyzed with a cationic ion-exchange column (Theoquest Hypersil SCX, Thermo), and the absorbance was measured at 206 nm.