| Â | Column 1 | Column 2 | Column 3 | Column 4 | Column 5 | Column 6 | Column 7 |
|---|
Selection Medium | Neo + Hygro | Neo | Hygro | TS Phenotype | NON-TS Phenotype (Neo) | NON-TS Phenotype (Hygro) | TS rate per 1 kb |
|---|
Experiment 1 | 3,200 | 7,000 | 21,000 |
6,400
|
3,800
|
17,800
|
22.9%
|
Experiment 2 | 4,300 | 16,500 | 22,500 |
8,600
|
12,200
|
18,200
|
22.1%
|
Experiment 3 | 600 | 4,100 | 1,400 |
1,200
|
3,500
|
800
|
21.8%
|
average | Â | Â | Â | Â | Â | Â |
22.3%
|
- KG81 and KG82 (Fig. 2A) were combined and packaged in 293T cells. Recipient HEK 293 cells were transduced with vector preparations and cells were grown on culture plates supplemented with either neomycin, hygromycin, or both. Colonies were quantified. Column1: number of colonies counted on plates with Neomycin and Hygromycin; column 2: number of colonies counted on plates with Neomycin; column 3: number of colonies counted on plates with Hygromycin; column 4: TS phenotypes (including the double sensitive phenotype that does not grow on the double antibiotics plate) calculated as 2× column 1 (e.g. 2 × 3,200 = 6,400); column 5: Non-TS phenotypes (NeoR, HygroS) calculated as column 2 - column 1 (e.g. 7,000 - 3,200 = 3,800); column 6: Non-TS phenotypes (NeoS, HygroR) calculated as column 3 - column 1 (e.g. 21,000 - 3,200 = 17,800); column 7: TS rate within 1 kb fragment calculated as 100×(column 4/[column 4 + column 5 + column 6]) (e.g. 100 × (6400/[6400+3800+1780]) = 22.9%).
