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Figure 6 | Retrovirology

Figure 6

From: Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 HIV-1 strains ex vivo

Figure 6

Early HIV-1 NL4-3 gene expression in infected T-cells from individual hCycT1-tg rats is variable, and the degree of enhancement does not correlate with hCycT1 steady-state levels. (A) T-cells derived from 10 hCycT1-tg and four n-tg rats were infected with VSV-G HIV-1NL4-3 GFP and analyzed three days later as described in the legend to Fig. 3. Levels of CD4 T-cells in these cultures ranged from 7 to 26% (and 74 to 93% CD8 T-cells). The MFI of all GFP-expressing, infected cells was determined, and the arithmetic mean ± SD of triplicates is given for one experiment. At the time of HIV-1 infection, uninfected T-cells were harvested for (B) western blot analysis to assess the expression of hCycT1, with ERK2 serving as a loading control.

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Retrovirology

ISSN: 1742-4690

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