Figure 9

TAR 5' miRNA targeted the ERCC1 gene directly. (A) miRanda software was used to determine potential target sites in the ERCC1 and IER3 mRNA sequences for the HIV-1 TAR 5' miRNA. (B) 293T cells were transfected with TAR-D or TAR-WT RNA. Twenty-four hours after RNA transfection the cells were transfected with psiCheckERCC-737 reporter vector. Extracts were prepared and luciferase expression determined at 24, 48 and 72 hours after reporter transfection. Data shown represent the normalized expression of Renilla luciferase (with the target region) to firely luciferase. Data are representative of two replicates.