The effect of imp7 KD on HIV-1 infection is independent of cell-cycle arrest. (A) HeLa cells analyzed by FACS after staining with propidium iodide. Top panel, dot plot of forward and side scatter; the gated population was used for cell cycle analysis, middle panel, no aphidicolin, bottom panel, cells treated with 1.5 micrograms/ml aphidicolin. M1, G1 phase; M2, S phase; M3, G2/M phase. (B) FACS analyses of HeLa DxR and imp7 KD cells infected at an MOI of 0.03 with a VSV-G pseudotyped HIV-1 vector (pCSGW), SIVmac vector and HIV-2 vector in the absence or presence (Aph) of aphidicolin. Cells were analyzed for GFP expression 20–24 hours post infection. Bars represent the mean value ± SD of three independent experiments. Infection levels with an MLV vector were 10 fold lower in aphidicolin-treated cells compared to control cells (not shown).