Figure 6

Flow cytometric sorting strategy for monocytes and T cells. A representative exemple is shown. PBMCs from each animal were stained with the antibody combination described in the material and methods. Monocytes and lymphocytes were defined with forward and side scatter (I). CD3+ T cells were then defined based on expression of CD3 (II). CD4+ T cells were then defined based on expression of CD4 without expression of CD8 (III). Naïve CD4+ T cells were then separated based on expression of CD28 without expression of CD95 (IV). Central memory CD4+T cells were then separated based on dual expression of CD28 and CD95 (IV). Effector memory CD4+ T cells were then separated based on expression of CD95 without expression of CD28 (IV). The CD14+ monocytes were separated based on expression of CD14+ (II').