Figure 4

Expression of SCTs of RT1.A^l fused with EGFP. (A) Diagram of SCTs of RT1.A^l fused at its C-terminal end to EGFP. (B and C) The 293T cells were either untreated or transfected with pEF/RT1Al-EGFP, pEF/RT1AlSCNLEnv371L-EGFP, or pEF/RT1AlSCTax180L-EGFP. After 48 hours of transfection, the 293T cells were incubated with 4O1/C8 cells for 24 hours. Production of IFN-γ (B) and TNF-α (C) in the supernatants was measured by ELISA. For 293T/RT1Al-EGFP cells, NLEnv371-379 or Tax180-188 peptides were pulsed for 30 min before the mixed culture with 4O1/C8. The data represent the mean ± the SD of triplicate wells. Similar results were obtained in two independent experiments.