Figure 5

The effect of D4T and β-Estradiol on the replication of a D4T-resistant mutant of HIV. PBL were infected in parallel with wild type HIV-1 (strain 9320), open, or D4T-resistant HIV-1 (HIV-1-D4Tr), shaded, and were grown in the presence of either no added drug or hormone, 50 nM D4T alone, 2 nM β-estradiol alone, or the two together. Supernatants were harvested for p24 analysis on day 7. In each experiment infected cells were plated in triplicate wells of a 24 well plate, each containing 2 million PBL, and the average replication determined normalized to virus alone ("VA). The data presented represent the averages of three independent experiments on independent donors. The error bars represent +/- one SD.