Figure 1

Schematic drawings of the APOBEC3G-carrying plasmids. E: untagged APOBEC3G-carrying plasmid (pcDNA3.1(-)-Apo-E/Hygromycin); U: same plasmid but contains an in-frame fusion of UB2A with APOBEC3G (pcDNA3.1(-)-Apo-U/Hygromycin); M: same as U but contains an in-frame fusion of a mutated UBA2* with APOBEC3G (pcDNA3.1(-)-Apo-M/Hygromycin). The asterisk * by UBA2 indicates location of a single point mutation in the UBA2 domain (L392A) that renders it incapable of stabilizing proteins [35]. P_CMV, CMV promoter; the single letter restriction enzyme designations are: X, XhoI; E, EcoRI; H, HindIII.