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Table 1 Summary of the HIV-1 envelopes tested in the different experimental models.

From: HLA-C increases HIV-1 infectivity and is associated with gp120

Experimental model

Host cell

(HLA-C allele)

Env

(tropism/subtype)

HLA-C siRNA silencing and cell

fusion assays

HeLa (Cw12)

ADA (R5/B)

LAI (X4/B)

NDK (X4/D)

gp120/gp41 transient transfection

and cell fusion assays

CHO-HLA-C (Cw4)

93MW965 (R5/C)

91US005 (R5/B)

92UG024 (X4/D)

NDK (X4/D)

J500 (X4/B)

Pseudovirus transductions

293T (Cw7)

pRHPA4259.7 (R5/B)

6535.3 (R5/B)

NDK (X4/D)

m7NDK (X4/D)

  1. HLA-C silencing was conducted on human cells (HeLa-derived) physiologically expressing HLA-C and stably expressing Env of different strains (ADA, LAI, NDK).
  2. Transient transfections experiments with plasmids encoding different Envs were conducted on non-human CHO cells stably expressing HLA-C to directly compare the effect of HLA-C in the absence of other human MHC class I molecules.
  3. Pseudoviruses were produced in HLA-C silenced 293T cells since this human cell line is the election host for efficient and quantitative production of pseudotyped virus particle. The Envs tested belong to a standard reference panel (NIBSC EVA CFAR ARP2066) except NDK.
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Retrovirology

ISSN: 1742-4690

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