Figure 4

Effect of blood and culture T. cruzi trypomastigotes and 24 h-supernatants of trypomastigotes on HIV-1 replication in placental histocultures. A: Placental villi were transducted overnight with BaL (B) (250 ng p24/placental block) or VSV-G pseudotyped HIV-1 (V) (100 ng p24/placental block) alone or in the presence of blood trypomastigotes (BT) (10⁶ parasites/placental block). Fragments were homogenized and luciferase activity as an indicator of viral replication was read from tissue lysate at day 4 post-infection or coinfection. Results are expressed as relative light units per second (RLU/sec), presented as a percentage relative to B or V and were normalized in each sample by total protein concentration (RLU/prot). The histogram in red corresponds to the % of infection with BaL or VSV-G (= 100%) and the histogram in white corresponds to the % of infection in the presence of BT. B: Placental villi were transducted overnight with VSV-G pseudotyped HIV-1 (V) (100 ng p24/placental block) alone or in the presence of blood trypomastigotes (BT) or cell trypomastigotes (CT) (10⁶ parasites/placental block), or 24 h-supernatants of BT (BTSn) or 24 h-supernatants of CT (CTSn). Fragments were homogenized and luciferase activity as an indicator of viral replication was read from tissue lysate at day 4 post-infection or coinfection. Results are expressed as relative light units per second (RLU/sec), presented as a percentage relative to V and were normalized in each sample by total protein concentration (RLU/prot). The histogram in red corresponds to the % of infection with VSV-G (= 100%) and the histogram in white corresponds to the % of infection in the presence of BT, CT, BTSn or CTSn (p < 0.001). Results are represented as a mean of 5 independent experiments.